Two Brothers may refer to:
Film and television
- Two Brothers (1929 film), a German silent film directed by Mikhail Dubson
 - Two Brothers (2004 film), a French-British film directed by Jean-Jacques Annaud
 - The Two Brothers (film), a 1910 American short Western film directed by D. W. Griffith
 - Two Brothers Pictures, a British television production company founded in 2014
 
Literature and folklore
- Tale of Two Brothers, an ancient Egyptian story
 - The Two Brothers, a German fairy tale
 - The Two Brothers (novel), or La Rabouilleuse, an 1842 novel by Honoré de Balzac
 - Dois Irmãos (Two Brothers), a 2000 novel by Milton Hatoum
 
Music
- "The Twa Brothers", a traditional ballad
 - Two Brothers (album), a 2001 album by Boxhead Ensemble
 
Places
- Two Brothers, U.S. Virgin Islands, a settlement on the island of Saint Croix
 - Two Brothers Rocks, a rock formation in the Ionian Sea near Syracuse, Sicily
 
Other
- Two Brothers (ship), a 19th-century Nantucket whaleship
 - Two Brothers (video game), a 2013 action role-playing game
 - Two Brothers Brewing, an American microbrewery
 - Two Brothers Roundhouse, on the former Chicago & Aurora Railroad, in Aurora, Illinois, U.S.
 
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